The deliverable evaluates the communication transport protocols for the selected traffic sources in different rail scenarios. Considered transport protocols are TCP with cubic and BBR versions, UDP and SCTP while sources are CBR, VBR and file transfer. The analysis is implemented in three realistic rail scenarios served by LTE or WiFi: the Roma –Firenze high-speed line (as mainline environment); the Roma –Firenze regional line (as regional/freight line); Metro of Rome (as metro/urban line).